Contact an Account ManagerExtraction of THC and 11-nor-9-THC-narboxylic acid from urine
Summary
Type: SPE
Application #: 303910
Catalog#: 730680 - SPE Cartridges, Chromabond, Drug II, 3ml, PP, 200mg, 50/pk
Detection: Derivatisation with MSTFA (Cat.No. 701270)
GC-MS on OPTIMA® 5 MS:
25 m, 0.2 µm, 0,2 mm ID (Cat.No. 726210.25) or
12 m 0.35 µm, 0.2 mm ID (Cat.No. 726215.12):
Temp.: 150°C hold for 1 min; 9 °C/
Conditions: Column conditioning: 3 ml methanol, followed by 3 ml 0.1 M acetate buffer (pH 7)
Sample application: aspirate slowly the sample through the column without vacuum (1-2 ml per min)
Column washing: elute interferences with 3 ml acetate buffer (pH 4, 0.1 M) – MeOH (1:1, v/v) followed by 3 ml water – methanol (1:1, v/v); dry the sample 5 min in vacuum
Elution: elute THC and metabolites with 1.5 ml acetone – glacial acetic acid (99:1, v/v)
Authors: Köhler H
Source: Universitätsklinikum Münster Inst Fü
Details
Legend: Recovery rates: 70 - 80%